CELL CULTURE SERA & MEDIA • 3D culture and complex cell models CORNING® Corning® ULA Treatment 1 and 2 Corning® ELPLASIA® Technology 2 Corning® Plates MATRIGEL® 3D Coated 3 GREINER BIO-ONE CELLSTAR Cell-Repellent Treatment 3 GREINER MAGNETIC 3D Cell Culture M3D Technology 4 and 5 Imaging system 5 HCS PHARMA Biomimesys 3D technology 6 Biorcell3D Artificial® Tumour Kit 7 Cell culture media 8 NUNCTM 10 SPL 10 Extracellular matrices - Technical solutions for dissociations - Clearing 11 to 13 Culture inserts 14 to 22 Ivtech solutions 23 • Adherent cells culture Cell culture flasks 24 à 26, 28, 30, 32 Culture chambers 27, 29, 31 Petri dishes 33 to 37 Coated petri dishes 37 Petri dishes with glass bottom 38 Cloning cylinders 39 Culture plates and microplates 39 to 44 Coated Culture plates 45 and 46 Plates for cell imaging 46 to 48 • Suspension cells culture Cell culture flasks 49 to 51 Petri dishes 52 Culture plates and microplates 53 and 54 Bioreactors 54 Culture Erlenmeyer 55 to 57 Spinners and stirring flasks 58 and 59 Rollers 60 to 62 Culture pouches 62 • The essentials in vitro fertilisation materials 63 Culture holders for enbryos, culture chambers 64, 64 to 67 Slide culture flasks 68 Culture Slides and coverslips 69 Centrifuge tubes15 et 50 ml 70 to 74 Large volume centrifuge tubes 75 and 76 Culture tubes 76 to 81 Cell scrapers 82 to 83 Cell strainers 84 to 86 Grinders 87 to 93 Small cell culture equipment : measuring equipment 93 Plant cultivation 94 and 95 - Animal and human sera, sera with treatment, FBS GOOD, plasma, FetalClone, BSA, PAN BIOTECH, DUTSCHER, HYCLONE, ID BIO, CORNING 96 to 98 • Culture media DMEM, MEM, RPMI, GLASCOW, HAM’S, IMDM, BME, CMRL, M199, Leibovitz’s, Mc COY’S, WILLIAMS E 99 to 104 • Buffered saline solutions : EBSS, HBSS, HEPES, DPBS, Tryode, Puck, GEY’S, PBS, SSC, TAE 105 and 106 • Amino acids, vitamins, additives 107 • Antibiotics and antimycotics 108 • Cell dissociation products 109 and 110 • Cryoconservation 110 • Growth factors 111 • Mycoplasma prevention, detection and treatment 111 and 112 • Specific media : AMNIOPAN, ENDOPAN, EMEM, MARROWPAN, NEUROPAN, PANTUM 113 • Serum substitutes : PANEXIN, NU-SERUM 113 and 114 • Reduced serum media 114 • Insect media 115 • Cell culture water 116 • Sterile, non-pyrogenic water, sodium chloride 117 • Medium without serum : PANSERIN, HybriGro SF™ 118 and 119 • Stem cell media 120 • Extra-cellular matrices 120 • Media and Plant Culture in vitro 121 to 123 • Preparation of blood samples 124 and 125 • BIOPREDIC Cells 126 • PluriBeads Microbeads 127 An extraordinary adventure
Cell Culture 1 Indicative prices. Visit www.dulis.nl 3D culture and complex cell models The different methods in cell culture Advantages hEasy to handle as it has been mastered for a long time hEasy observation of cells hSimple environmental control Disadvantages hInteractions between different cells in our body, are not glued flat to each other hDoes not create a real microenvironment because the cells in our body organise themselves differently hFlat cells are not equally resistant to drugs Advantages hReal cell-cell interactions hTrue interactions between cells and the biochemical and physiological microenvironment hOptimal diffusion of gradients (oxygen, nutrients, waste...) hDifferent reaction to stimuli (temperature, pH...) Disadvantages hMore complex culture method, but this is fading with the development of new media hHarder cell observations: more suitable consumables and transparency techniques now improve these observations Consequences hAnalyses and tests are not very predictive because 2D cells have a different morphology and phenotype than in vivoconditions hSome drugs that are ineffective in 2D would have been effective in 3D and vice versa hUse of numerous animal tests to validate the first 2D results Consequences hDrug analyses and tests are becoming much more predictive hBetter representation of the real (physiological) conditions of our body hStudy of drug resistance in a more physiological way, with cells forming a real barrier hBetter screening of molecules of interest; less animal testing hTime and financial savings A traditional method used for decades, the cells are mainly deposited on a flat substrate and therefore form flat layers. Once you want to go into 3D, what’s next? - You are patient, you like to read and you leaf through the following pages which will guide you in the choice of different media to get there. - If you are impatient and want to move forward quickly, you can contact our team of cell culture specialists directly, who will analyse your scientific project and technical needs and offer you the best solutions to get there. A new method of culture where the cells are organised differently because the different methods used allow them to avoid lying flat. 2D cell culture method 3D cell culture method h Your specialist contacts Romain GUEGAN romain.guegan@dulis.be Marie OSTERTAG marie.ostertag@dulis.be 2D 3D
Cell Culture 2 ULA rounded-well microplate Squared well Plasma Microplate Solutions provided by Corning® hUltra-Low Attachment (ULA) surfaces have a hydrogel layer that minimises cell attachment, protein uptake and enzyme activation (the surface is non-cytotoxic, biologically inert, and non-degradable) hSterile h Applications h3D formation of spheroids hThe maintenance of cells in suspension hPreventing stem cell differentiation hPreventing the division of adherent cells hOne plate for all steps (culture and testing), no handling or delicate transfer of spheroids required hOptically clear round bottom well hUltra-Low Attachment: surface treatment with very low cell adhesion hUnique well geometry for reproducible well-to-well growth of 3D cultures hSterile hEnables mass production of uniformly sized spheroids for in vivo tumour studies or screening of anti-cancer molecules hTwo well geometries: rounded (for spheroid formation and expansion) or squared (more suitable for image analysis) hThe bottom of the wells have micro-cavities allowing the formation of several spheroids per well; up to 15 796 spheroids per 1 well hULA or PLASMA treatment depending on the model hSupplied with cap hUnit packaging hSterile Corning® Treatment ULA (Ultra-Low Attachment) Corning® ULA treatment and a specific design for spheroids Corning® ELPLASIA® technology for mass production of spheroids Cat. No. Number of wells Colour Treatment Type of bottom Units/ bag Units/ box €/ box 003471 6 Transparent ULA Flat 1 24 N/A - 003473 24 Transparent ULA Flat 1 24 N/A - 007007 96 Transparent ULA Rounded 1 24 N/A - 003474 96 Transparent ULA Flat 1 24 N/A - 004588 384 Black ULA Flat-Transparent 20 100 N/A - Cat. No. Number of wells Treatment Colour Type of bottom Units/ bag Units/ box €/ box 004520 96 ULA Black Rounded-Transparent 10 50 N/A - 004515 96 ULA Black Rounded-Transparent 1 5 N/A - 003830 384 ULA Black Rounded-Transparent 10 50 N/A - 003830BC 384 ULA Black Rounded-Transparent 10 50 N/A - 004516 384 ULA Black Rounded-Transparent 1 5 N/A - 004637 1536 ULA Black Rounded-Transparent 1 5 N/A - 004527 1536 ULA Black Rounded-Transparent 10 50 N/A - Cat. No. Number of wells Treatment Colour Type of bottom Average number of spheroids/well Units/ bag Units/ box €/ box Rounded wells 004440 6 ULA Black Flat-Transparent 2885 1 5 N/A - 004441 24 ULA Black Flat-Transparent 554 1 5 N/A - 004442 96 ULA Black Flat-Transparent 79 1 5 N/A - Squared wells 004444 6 PLASMA Black Flat-Transparent 15796 1 5 N/A - 004445 24 PLASMA Black Flat-Transparent 2934 1 5 N/A - 004446 96 PLASMA Black Flat-Transparent 475 1 5 N/A - 004447 384 PLASMA Black Flat-Transparent 137 1 5 N/A - Cat. No. Type of Flask Type of cap Treatment Units/ bag Units/ box €/ box 003814 75 cm2 Filter ULA 4 24 N/A - 003303 CellSTACK® 1 Level 636 cm2 Filter ULA 1 8 N/A - Cat. No. Diameter (mm) Treatment Units/ bag Units/ box €/ box 003261 60 ULA 5 20 N/A - The plates Flasks Petri dishes 003830 004520 004637
Cell Culture 3 All prices are indicative and exclude VAT. Visit www.dulis.nl Solutions provided by Corning® / Greiner BIO-ONE hCulture plates coated with 3D-qualified Matrigel® hIdeal for screening research on spheroids, organoids, or tumoroids hImprove the repeatability and accuracy of 3D analyses Corning® Plates MATRIGEL® 3D Coated Cat. No. Number of wells Treatment Colour Type of bottom Units/ bag Units/ box €/ box 356259 96 Matrigel® without phenol red Black FlatTransparent 1 1 N/A - 356256 384 Matrigel® without phenol red Black FlatTransparent 1 5 N/A - 356257 384 Matrigel® without phenol red White FlatTransparent 1 5 N/A - 356258 384 Matrigel® without phenol red White FlatTransparent 1 1 N/A - CELLSTAR® Cell-Repellent Treatment hSterile hNon-cytotoxic h The CELLSTAR® Cell-Repellent surface treatment of Greiner Bio-One effectively prevents cell adhesion hSpheroid and aggregated stem cell cultures hSuspension culture of adherent or semi-adherent cells hCulture on hydrogel or equivalent h3D magnetic culture with the M3D Greiner Bio-One system hThanks to an innovative chemical modification of the polymer, the Cell-Repellent Greiner Bio-One surface does not interfere with culture conditions, making it an excellent support for primary or sensitive cultures hUnit packaging possible on certain items, please consult our specialist DNase RNase Human DNA Endotoxins Cat. No. Number of wells Treatment Colour Type of bottom Units/ bag Units/ box €/ box 657970 6 Cell-Repellent Transparent Flat 1 5 N/A - 665970 12 Cell-Repellent Transparent Flat 1 5 N/A - 662970 24 Cell-Repellent Transparent Flat 1 5 N/A - 677970 48 Cell-Repellent Transparent Flat 1 5 N/A - 655970 96 Cell-Repellent Transparent Flat 1 6 N/A - 650970 96 Cell-Repellent Transparent Rounded 1 6 N/A - 650979 96 Cell-Repellent Transparent Rounded 8 32 N/A - 651970 96 Cell-Repellent Transparent V 1 6 N/A - 655976 96 Cell-Repellent Black Flat-Transparent 8 32 N/A - 781970 384 Cell-Repellent Transparent Flat 1 60 N/A - 787979 384 Cell-Repellent Transparent Rounded 8 32 N/A - 781976* 384 Cell-Repellent Black Flat-Transparent 8 32 N/A - * µClear bottom Cat. No. Type of Flask Treatment Type of bottom Units/ bag Units/ box €/ box 690980 25 cm2 Cell-Repellent Standard 10 20 N/A - 690985 25 cm2 Cell-Repellent Filter 10 20 N/A - 658980 75 cm2 Cell-Repellent Standard 5 15 N/A - 658985 75 cm2 Cell-Repellent Filter 5 15 N/A - 660980 175 cm2 Cell-Repellent Standard 5 5 N/A - 660985 175 cm2 Cell-Repellent Filter 5 5 N/A - High shape 661980 175 cm2 Cell-Repellent Standard 4 4 N/A - 661985 175 cm2 Cell-Repellent Filter 4 4 N/A - Cat. No. Diameter (mm) Treatment Units/ bag Units/ box €/ box 627979 35 Cell-Repellent 10 40 N/A - 628979 60 Cell-Repellent 10 20 N/A - 664970 100 Cell-Repellent 1 5 N/A - Plates Flasks Petri dishes
Cell Culture 4 Solutions provided by GReiner BIO-ONE Greiner Bio-One 3D magnetic technology / M3D Cell Culture Cat. No. Format Description Content € the kit 657840 6 wells 6-well Bioassembler™ kit - 1 x Levitating Drive: holder for levitated spheroids formation - 1 x Holding Drive: holder for spheroids - 2 x Nanoshuttle™ -PL magnetic beads, 600 µl tube - 2 x 6-well Cell-Repellent plate Cat.No. 657970 - 2 x Special intermediate cover Cat. No. 657825 N/A - 662840 24 wells 24-well Bioassembler™ kit - 1 x Levitating Drive: holder for levitated spheroids formation - 1 x Holding Drive: holder for spheroids - 2 x Nanoshuttle™ -PL magnetic beads, 600 µl tube - 2 x 6-well Cell-Repellent plate Cat.No. 662970 - 2 x Special intermediate cover Cat. No. 662825 N/A - Cat. No. Format Description Content € the kit 655840 96 wells 96-well magnetic bioprinting kit 1 x Spheroid Drive: holder for bioprinting spheroids formation 1 x Holding Drive: holder for spheroids 2 x Nanoshuttle™ -PL magnetic beads, 600 µl tube 2 x Cell-Repellent transparent 96-well plate Cat.No. 655970 N/A - 655841 96 wells 96-well black magnetic bioprinting kit 1 x Spheroid Drive: holder for bioprinting spheroids formation 1 x Holding Drive: holder for spheroids 2 x Nanoshuttle™ -PL magnetic beads, 600 µl tube 2 x Cell-Repellent µclear bottom black 96-well plate Cat.No. 655976 N/A - 073054 384 wells 384-well magnetic bioprinting kit 1 x Spheroid Drive: holder for bioprinting spheroids formation 1 x Holding Drive: holder for spheroids 2 x Nanoshuttle™ -PL magnetic beads, 600 µl tube 2 x Cell-Repellent transparent 384-well plate Cat.No. 781970 N/A - 781841 384 wells Black 384-well Magnetic BioPrinting Kit 1 x Spheroid Drive: holder for bioprinting spheroids formation 1 x Holding Drive: holder for spheroids 2 x Nanoshuttle™ -PL magnetic beads, 600 µl tube 2 x Cell-Repellent µclear bottom black 384-well plate Cat.No. 781976 N/A - Cat. No. Description Units/ bag Units/ box €/ box 657825 Special intermediate lid 6 wells 2 10 N/A - 662825 Special intermediate cover 24 wells 2 10 N/A - Magnetic levitation Magnetic bioprinting Ring drive 96 wells and 384 wells for Magnetic bioprinting Intermediate lids alone hSystem for 3D cell culture using transient magnetization of cells that naturally attach to NanoShuttleTM-PL magnetic beads hThanks to the magnetic force, the cells aggregate, either by levitation or by bioprinting, to form 3D representation models in vitro hReproduction of native tissue environments in vitro hVersatile system compatible with all cell types even in co-culture h3D model formation within a few hours after incubation with magnetised beads hReproducible and usable up to 1536 well format hNo impact of the magnetised beads on the cells (proliferation, viability, phenotype, etc.) or on the analyses (qPCR, cytometry, fluorescence microscopy, western blot, etc.) hCentred positioning of spheroids hNo specialised equipment, media or artificial substrate hEasy to handle, no loss of sample during washing, transfer or labelling of spheroids/organoids hCan be used as a complementary tool, compatible with a large number of 3D models and consumables Cat. No. Format € 655850 96 wells N/A - 073062 384 wells N/A - Allow physical separation of cells and magnets. Magnetic-Ring Stand for 96 or 384 well plates.
Cell Culture 5 All prices are indicative and exclude VAT. Visit www.dulis.nl Solutions provided by GReiner BIO-ONE Greiner MAGNETIC 3D Cell Culture M3D Technology (continued) Magnetic-ring printing and imaging system Cat. No. Format Description Content € the kit 655846 96 wells BIOASSAY™ 96-well kit - 1 x Spheroid Drive: holder for bioprinting spheroids formation - 1 x Levitating Drive: holder for levitated spheroids formation - 1 x Holding Drive: holder for spheroids - 1 x Guide 96-well for magnetic bioprinting Cat.No. 655850 - 3 x Nanoshuttle™ -PL magnetic beads, 600 µl tube - 1 x Deepwell 96 well - 2 x Cell-Repellent transparent 96-well plate ref. 655970 - 2 x Cell-Repellent 6-well plate Cat.No. 657970 N/A - 655849 96 wells BIOASSAY™ 96-well kit with imaging system Identical to Cat.No. 655846 with imaging system Cat.No. 657860: - 1 x n3Dock - 1 x Imaging system - 1 x Light pad - 1 x Fan - 1 x AD-USB adapter - 1 x extension - 1 x Software N/A - 781846 384 wells BIOASSAY™ 384-well kit - 1 x Spheroid Drive: holder for bioprinting spheroids formation - 1 x Levitating Drive: holder for levitated spheroids formation - 1 x Holding Drive: holder for spheroids - 1 x Guide 96-well for magnetic bioprinting Cat.No. 655850 - 2 x Nanoshuttle™ -PL magnetic beads, 600 µl tube - 1 x Deepwell 96-well - 2 x Cell-Repellent transparent 384-well plate Cat. No. 781970 - 2 x Cell-Repellent 6-well plate Cat. No. 657970 N/A - 073055 384 wells BIOASSAY™ 384-well kit with imaging system Identical to Cat.No. 781846 with imaging system Cat.No. 657860: - 1 x n3Dock - 1 x Imaging System - 1 x Light pad - 1 x Fan - 1 x Adapter AD-USB - 1 x Extension - 1 x Software N/A - Magnetic pen for individual handling of formed spheroids. M3D imaging system and consumables Nanoshuttle™ -PL magnetic beads MagPen Cat. No. Description Content € the kit 657860 Imaging system kit - 1 x n3Dock - 1 x imaging system - 1 x Light pad - 1 x fan - 1 x adapter AD-USB - 1 x extension - 1 x software N/A - Cat. No. Description Units/ bag Units/ box €/ box 657841 Nanoshuttle™ -PL magnetic beads, 600 µl tube 1 1 N/A - 657843 Nanoshuttle™ -PL magnetic beads, 600 µl tube 1 3 N/A - 657846 Nanoshuttle™ -PL magnetic beads, 600 µl tube 1 6 N/A - 657852 Nanoshuttle™ -PL magnetic beads, 600 µl tube 1 12 N/A - Cat. No. Description Units/ bag Units/ box €/ box 657850 MagPen magnetic pens 1 3 N/A - (2) (1) Our solutions, your results (3) (4) (1) Ravi Birla, and al. Assembly of a functional 3D primary boxiac construct using magnetic levitation, AIMS Bioengineering, 3(3): 277-288 (2016) (2) Leenhardt, R., Camus, M., Mestas, J.L. and al. Ultrasound-induced Cavitation enhances the efficacy of Chemotherapy in a 3D Model of Pancreatic Ductal Adenocarcinoma with its microenvironment. Sci Rep 9, 18916 (2019). (3) Noel, P., Muñoz, R., Rogers, G. W., Neilson, A., Von Hoff, D. D., Han, H. Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts. Vis. Exp. (126), e56081, doi:10.3791/56081 (2017). (4) 25 and 50 µg/ml - Hau, H., Khanal, D., Rogers, L., Suchowerska, N., Kumar, R., Sridhar, S., McKenzie, D. and Chrzanowski, W. (2016), Dose enhancement and cytotoxicity of gold nanoparticles in colon cancer cells when irradiated with kilo‐ and mega‐voltage radiation. Bioengineering & Translational Medicine, 1: 94-102.
Cell Culture 6 Biomimesys 3D technology TIP Our partner, HCS PHARMA, is also available to study any form of customisation based on this hydroskeleton. Please do not hesitate to contact us. Solutions provided by HCS PHARMA A 3D microenvironment adapting to the different stiffness and compositions of the matrices within tissues and organs. Biomimesys 3D hydroscaffolds are all hyaluronic acid based. This hydroscaffold provides a tailor-made support, controlled and physiologically adapted to the cells in which they rest. h Advantages of this hydroscaffold • Exchange improved circulation of gases and nutrients • Interactions: optimal cell-cell and cell-matrix interactions • Controlled composition: precision of the components introduced, guaranteeing reproducibility of measurements • Ready to use: easy to seed, freeze-dried matrix in the plate • Translucent: ideal for microscopy and plate readers (screening) • Porous: between 100 and 150 µm; rapid impact of lysis buffer, ideal for PCR, Western Blot and ELISA • Biodegradable: easy to dissociate by specific enzymes, ideal for flow cytometry • Solid: for long-term culture, ideal for histology and in vivo implantation Composition: Hyaluronic acid + collagen I hPromotes 3D structure: spheroids or organoids hFacilitates variation in spheroid shape and size depending on: cell density, culture time and cell type hImproves motility, proliferation and migration hAvoids immune reactions in in vivo implantations Composition: Hyaluronic acid + RGDS peptide + collagen I + collagen VI hPromotes 3D structure: spheroids or organoids hEnsures differentiation of pre-adipocytes into mature adipocytes hImproves lipid accumulation during growth (maturation indicator) hIncreases expression of adipogenesis marker genes Composition: Hyaluronic acid + RGDS peptide + Galactosamine + collagen I + collagen IV hFavours 3D structure: spheroids or organoids for metabolic or toxicity research hPreserves the metabolic activity of primary human hepatocytes Composition: Hyaluronic acid + RGDS peptide + collagen IV + Cationic Biopolymer hPromotes 3D structure: spheroids or neural networks hProlongs culture in the best conditions Cat. No. Description Units/ box €/ box 159100 Transparent 96-well plate with 96 hydroscaffolds 1 N/A - 159101 Transparent 96-well plate with 24 hydroscaffolds 1 N/A - 159102 Black 96-well plate, transparent bottom with 96 hydroscaffolds 1 N/A - 159103 Black 96-well plate, transparent bottom with 24 hydroscaffolds 1 N/A - 159118 Black 384-well plate, transparent bottom and 384 hydroscaffolds 1 N/A - 159119 Black 384-well plate, transparent bottom and 384 hydroscaffolds 5 N/A - Cat. No. Description Units/ box €/ box 159104 Transparent 96-well plate with 96 hydroscaffolds 1 N/A - 159105 Transparent 96-well plate with 24 hydroscaffolds 1 N/A - 159106 Black 96-well plate, transparent bottom with 96 hydroscaffolds 1 N/A - 159107 Black 96-well plate, transparent bottom with 24 hydroscaffolds 1 N/A - Biomimesys oncology: for optimal 3D cancer cell culture Biomimesys liver: for optimal 3D culture of hepatocytes Biomimesys brain: for an optimal 3D culture of neuronal cells Biomimesys adipose tissue: for optimal 3D culture of adipocytes Cat. No. Description Units/ box €/ box 159108 Transparent 96-well plate with 96 hydroscaffolds 1 N/A - 159109 Transparent 96-well plate with 24 hydroscaffolds 1 N/A - 159110 Black 96-well plate, transparent bottom with 96 hydroscaffolds 1 N/A - 159111 Black 96-well plate, transparent bottom with 24 hydroscaffolds 1 N/A - 159116 Black 384-well plate, transparent bottom with 384 hydroscaffolds 1 N/A - 159117 Black 384-well plate, transparent bottom with 384 hydroscaffolds 5 N/A - Cat. No. Description Units/ box €/ box 159112 Transparent 96-well plate with 96 hydroscaffolds 1 N/A - 159113 Transparent 96-well plate with 24 hydroscaffolds 1 N/A - 159114 Black 96-well plate, transparent bottom witjh 96 hydroscaffolds 1 N/A - 159115 Black 96-well plate, transparent bottom witjh 24 hydroscaffolds 1 N/A -
Cell Culture 7 All prices are indicative and exclude VAT. Visit www.dulis.nl Solutions provided by Biorcell3D Artificial Tumour Kit® in ready-to-use microplates h Artificial tumours® (3D cultures) from tumour cell lines widely used in oncology hMore closely mimics the microenvironment of avascular tumours in vivo than 2D cultures hIncreasingly involved in the development of medicines hIdeal research solution for aggressive triple-negative breast cancer h The Opti3D® kit includes artificial tumours® produced in OptiPASS® culture medium in 96-well microplates (one tumour per well) hTransport in anoxia and at 4 °C to put in dormancy until use (alternative to freezing) h Each microplate contains 100 ml of suitable culture medium (OptiPASS® OPTI03) for in vitro testing h Advantages hDirect use of tumours after deconditioning hReproducible tumour size and morphology hGuaranteed: less than 10% intra- and inter-microplate size variation hOnce deconditioned, resumption of tumour growth for a minimum of 10 days hAvailable for triple-negative breast cancer, very aggressive subtype: MDA-MB-231 line, SUM1315 line hIdeal for screening, toxicity testing or basic research into mechanisms of interest Cat. No. Description Packaging € 631700 OPTI3D® SUM 1315 Kit 1 x 96-well microplate in anoxia N/A - 631701 OPTI3D® MDAMB 231 Kit 1 x 96-well microplate in anoxia N/A - TIP For other types of artificial tumours, please contact us Standard treatment used in this example: epirubicin LIVE/DEAD assay of MDA-MB-231 LIVE/DEAD cells Control not maintained Day 3 Day 5 Day 7
Cell Culture 8 Solutions provided by Biorcell3D Ready-to-use, complete, serum-free cell culture media h Advantages hReady to use hAbsence of compounds of animal origin hControlled composition hReproducibility hProlongation of viability and growth of cell models without change in phenotype or cell response hCultures can be preserved without freezing (in anoxia and at 4 °C) h 3 applications offered • 2D culture bioproduction • Oncology: 3D culture • Oncology: conservation OptiPASS media can be used for monolayer (2D) culture of tumour or healthy cells and for 3D culture of a wide range of tumour types of preclinical/clinical interest. hExample of VERO E6 cells, the OptiPASS® medium (OPTI04): - allows for cell proliferation and viability similar to serum culture - allows for preservation of cell phenotype - requires prior coating of culture plates hValidated tumour lines in 2D and 3D culture: - Breast: MDA-MB-231, SUM1315, BT474 - Prostate: PC-3 - Lung: NCI-H1975 - Ovary: SK-OV-3 hOptiPASS® medium (OPTI03) for artificial tumours®: - maintenance/increase of proliferation/growth rate - maintenance of metabolic gradients within cultures (live cells/dead cells) - reproducibility of production in terms of size and proliferation/growth h3D cultures can be stored for several days in OptiPASS® medium (OPTI05), under anoxic conditions and at 4 °C before analysis hExamples of tumour lines validated with this method: - Breast: MDA-MB-231, SUM1315 Cat. No. Description Volume (ml) € 631702 OptiPASS® OPTI04 50 N/A - 631703 OptiPASS® OPTI04 100 N/A - 631704 OptiPASS® OPTI04 500 N/A - Cat. No. Description Volume (ml) € 631705 OptiPASS® OPTI03 for 3D culture and Artificial ® Tumours 50 N/A - 631706 OptiPASS® OPTI03 for 3D culture and Artificial ® Tumours 100 N/A - 631707 OptiPASS® OPTI03 for 3D culture and Artificial ® Tumours 500 N/A - Cat. No. Description Volume (ml) € 631708 OptiPASS® OPTI05 for storage without freezing and in anoxia at 4 °C 50 N/A - 631709 OptiPASS® OPTI05 for storage without freezing and in anoxia at 4 °C 100 N/A - 631710 OptiPASS® OPTI05 for storage without freezing and in anoxia at 4 °C 500 N/A - 2D culture bioproduction Oncology: 3D culture Oncology: conservation Dubois and al, J Clin Med. 2019 Mar 21;8(3):397. doi: 10.3390/jcm8030397 Your specialist contact Romain GUEGAN romain.guegan@dulis.be Cell number Reference OptiPASS Proliferation rate per day Days of culture 3 days of culture Up to 7 days of conservation in OptiPASS® medium Culture Growth Restart for 10 days
Cell Culture 9 All prices are indicative and exclude VAT. Visit www.dulis.nl Dulis GOOD DEALS ON WWW.Dulis.nl
Cell Culture 10 Solutions provided by Nunc™ / Solutions provided by SPL NUNCLON™ SPHERA™ Treatment SPL3D™ Cell Floater treatment Nunc™ Nuclon™ Sphera™ plates allow for the optimization of sphere-shaped culture. They are suitable for different cell types. h Applications hBest model for studying tumour progression and the efficacy of anticancer agents, thanks to the reproduction of 3D structures during tumour growth hSuperior quality for the formation of embryoid bodies from pluripotent cells with minimal spontaneous differentiation hSterile Plates Flasks Petri dishes Cat.No. Number of wells Treatment Colour Type of bottom Units/ bag Units/ box €/ box 056864 6 NUCLON™ SPHERA™ Transparent Flat 1 7 N/A - 056863 12 NUCLON™ SPHERA™ Transparent Flat 1 7 N/A - 056862 24 NUCLON™ SPHERA™ Transparent Flat 1 7 N/A - 056861 96 NUCLON™ SPHERA™ Transparent Flat 1 8 N/A - 056883 96 NUCLON™ SPHERA™ Transparent Flat 5 50 N/A - 056860 96 NUCLON™ SPHERA™ Transparent Rounded 1 8 N/A - Cat.No. Type of Flask Treatment Type of cap Units/ bag Units/ box €/ box 056868 25 cm2 NUCLON™ SPHERA™ Filter 6 18 N/A - 056869 75 cm2 NUCLON™ SPHERA™ Filter 4 24 N/A - Cat. No. Diameter (mm) Treatment Units/ bag Units/ box €/ box 056865 35 NUCLON™ SPHERA™ 5 20 N/A - 056866 60 NUCLON™ SPHERA™ 5 20 N/A - 056867 90 NUCLON™ SPHERA™ 5 20 N/A - h3D cell culture media Plates hPromote the formation of animal cell spheroids hTreatment for low cell adhesion hIn polystyrene hSterile Cat. No. Diameter (mm) Treatment Units/ bag Units/ box €/ box 330186 35 SPL3D™ Cell Floater 10 20 N/A - 330187 60 SPL3D™ Cell Floater 10 20 N/A - 330188 90 SPL3D™ Cell Floater 10 10 N/A - Cat. No. Type of Flask Treatment Type of cap Units/ bag Units/ box €/ box 330193 25 cm2 SPL3D™ Cell Floater Filter 5 10 N/A - 330194 75 cm2 SPL3D™ Cell Floater Filter 1 3 N/A - Cat.No. Number of wells Treatment Colour Type of bottom Units/ bag Units/ box €/ box 330189 6 SPL3D™ Cell Floater Transparent Flat 1 5 N/A - 330190 24 SPL3D™ Cell Floater Transparent Flat 1 5 N/A - 330191 96 SPL3D™ Cell Floater Transparent Flat 1 5 N/A - 330192 96 SPL3D™ Cell Floater Transparent Rounded 1 5 N/A - Petri dishes Plates Flasks hPromotes the formation and development of spheroids hPolypropylene tube, HDPE cap, PET insert hHydrophobic PTFE filter membrane adhered to an air-permeable cap for improved gas exchange hInsert with 68 µm thickness and 65 µm porosity hDial at the cap for airflow control hSterile Spheroid formation unit Cat. No. Ø x h (mm) Total volume (ml) Units/ bag Units/ box €/ box 330195 17 x 120 15 3 90 N/A -
Cell Culture 11 All prices are indicative and exclude VAT. Visit www.dulis.nl Extracellular matrices - Technical solutions for dissociations - Transparisations Corning® Matrigel® Corning® Matrigel® Dissociation Corning® PURAMATRIX™ Solutions hBasal membrane extracted from a mouse sarcoma (EHS): rich in extracellular matrix proteins (laminin, collagen IV, entactin) and growth factors hPromotes the attachment and differentiation of many normal or transformed cells (hepatocytes, neurons, epithelial, endothelial...) hIn gel form, promoting 3D culture or allowing to measure the invasiveness of tumour cells hLDEV Free hTested negative for bacteria, fungi and mycoplasma hGel stability tested over 14 days at 37 °C hThe solution to improve your images and the analysis of your 3D structures hAllows visualization of the entire structure of spheroids, up to 500 µm in thickness hFull characterisation of the 3D model, allowing more accurate screening by visualising the core of the structure h Advantages • Fast clearing of tissues • No special equipment required • 3 to 4 times more cells detected • Compatible with Corning flat-bottom or round-bottom plates for spheroids • Compatible with fluorescence analyses, immunofluorescence analyses and numerous markers Cat. No. Description Packaging € 356255 Matrigel qualified for organoid culture, without phenol red 10 ml N/A - 354277 Matrigel qualified for human embryonic stem cells (hESC) 5 ml N/A - 356234 Matrigel with standard protein concentration 5 ml N/A - 354234 Matrigel with standard protein concentration 10 ml N/A - 356235 Matrigel with standard protein concentration 50 ml N/A - 354248 Matrigel with high protein concentration 10 ml N/A - 356237 Matrigel with standard protein concentration, without phenol red 10 ml N/A - 354262 Matrigel with high protein concentration, without phenol red 10 ml N/A - 356230 Matrigel with standard protein concentration, reduced in growth factors 5 ml N/A - 354230 Matrigel with standard protein concentration, reduced in growth factors 10 ml N/A - 354263 Matrigel with high protein concentration, phenol red free, reduced in growth factors 10 ml N/A - 356231 Matrigel with standard protein concentration, without phenol red, reduced in growth factors 10 ml N/A - Cat. No. Description Quantity € 354235 Dispase 100 ml N/A - 354253 Cell Recovery solution, non-enzymatic 100 ml N/A - Cat. No. Description Quantity € 005730 Corning 3D, Starter Kit, Cleaning Reagent, Antibodies, Blocking, Penetration and Washing Buffers 1 N/A - 005731 Corning 3D Clear Tissue Clearing Reagent 10 ml N/A - 005732 Corning 3D Clear Tissue Clearing Reagent 30 ml N/A - 005733 Corning 3D Clear Tissue Clearing Reagent 100 ml N/A - 005738 Corning, penetration buffer 30 ml N/A - 005739 Corning, penetration buffer 100 ml N/A - 005734 Corning, antibody buffer 30 ml N/A - 005735 Corning, antibody buffer 100 ml N/A - 005737 Corning, blocking buffer 100 ml N/A - Matrigel® cell detachment solutions. hSynthetic hydrogel peptide hPurified standard amino acid compound 1% (w/v) and 99% water hTransparent gel compatible with microscopic applications hFree of animal and pathogenic compounds hTested negative for bacteria and mycoplasma Cat. No. Volume € 354250 5 ml N/A - Corning® 3D Clear Solutions pour 3D culture clearing
Cell Culture 12 Extracellular matrices - Technical solutions for dissociations - Clearing Primary cells Cell-Tak™ Collagen I Collagen IV Matrigel® Fibronectin Gelatin Laminin Osteopontin Poly-Lysine (PDL, PLL) PDL/LM and PLO/ LM PuraMatrix® Vitronectin PureCoat™ ECM Mimetic Fn PureCoat ECM Mimetic COL I Synthemax® Surface Ultra-Low Attachment Osteo Assay Surface rLaminin-521 (Human) Primaria™ CellBIND® Surface PureCoat Amine PureCoat Carboxyl Extracellular matrices and biological coatings ECM Mimetics and Advanced Surface Improved CT surfaces Aortic endothelial cells, BAEC Epithelial cells of the bile duct x x Hepatocytes x x x x x x x x Cerebral microvessel cells (endothelial) xxx xxx x Cardiac cells (endothelium, progenitors), boxiomyocytes x x x x x x x x Colon Epithelial cells x x x Dorsal root ganglia x x x Embryonic cortical neurons x x Embryonic sympathetic neurons x x x x Endothelial cells x x x x x x Erythrocyte culture (stage of parasite development) x x Bone marrow cells, osteoclasts x Human periodontium (ligament) x Hippocampus Neurons x x x x x x HUVEC (endothelial) x x x x x x x x x HVSMC x x x Keratinocytes x x x x x x x Mammary epithelium Cells x x x x x Microvascular, BME (endothelial) x x x x x x Mouse splenic T cells x x x Muscle cells, myoblasts, myotubes, myogenic cells x x x Neuronal cells (cortical, cerebellar granule, astrocytes, sensory, sympathetic) x x x x x Oligodendrocytes (glia, precursors) x x x x Osteoblasts x x x Pancreatic islands, neonatal x x x x x Periodontium Acinar cells x x Peripheral blood mononuclear cells x x x x x x Schwann cells (glial) x x x x Sertoli cells x x Skeletal muscle cells (myocytes, myotubes) x x x Smooth muscle cells (endothelial, aortic, vascular) xxxx x x Urothelial cells xx x x hPromotes the formation and development of spheroids hThe collection of spheroids is facilitated hHyaluronic acid compound hReconstituted size: 280 µm hTotal weight: 1 g (0.2 g/tube) hSterile SPL matrix solution - Spheroid formation gel Cat. No. Description Units/ box €/ box 330196 Spheroid formation gel 5 tubes N/A - To find the best treatment for your cell lines, please contact our specialist Your specialist contact Romain GUEGAN romain.guegan@dulis.be How to choose the best matrix?
Cell Culture 13 All prices are indicative and exclude VAT. Visit www.dulis.nl Extracellular matrices - Technical solutions for dissociations - Clearing Corning® Extracellular and Synthetic Matrices Corning® Cell-Tak Corning® Synthemax™ II-SC hA solution for many cell culture problems hExtracellular matrix proteins improve adhesion, proliferation and differentiation of many cell types (hepatocytes, endothelial cells, neurons...) hThere are also directly coated culture plates (see "Adherent cell culture" range) Cat. No. Description Quantity € 354237 Extracellular matrix, human placenta 1 mg N/A - 354231 Collagen I, bovine skin 30 mg N/A - 354265 Collagen I, human placenta 10 mg N/A - 354243 Collagen I, human placenta 250 µg N/A - 354249 Collagen I, rat tail, high concentration 100 mg N/A - 354236 Collagen I, rat tail 100 mg N/A - 356236 Collagen I, rat tail 1 g N/A - 354257 Collagen II, bovine 5 mg N/A - 354244 Collagen III, human placenta 250 µg N/A - 354245 Collagen IV, human placenta 250 µg N/A - 354233 Collagen IV, mouse tumour 1 mg N/A - 356233 Collagen IV, mouse tumour 10 mg N/A - 354246 Collagen V, human placenta 250 µg N/A - 354261 Collagen VI human placenta 0.5 mg N/A - 354239 Laminin Ultra Pure without entactin 1 mg N/A - 354259 Laminin entactin complex, high concentration, mouse tumour 10.5 mg N/A - 354008 Fibronectin, human plasma 1 mg N/A - 356008 Fibronectin, human plasma 5 mg N/A - 356009 Fibronectin, human plasma 25 mg N/A - 354210 Poly D Lysine 20 mg N/A - 354238 Human Vitronectin 250 µg N/A - 354256 Osteopontin source human milk 100 µg/ml in PBS solution 50 µg N/A - Cat.No. Description Packaging € 354240 Corning Cell-Tak 1 mg N/A - 354241 Corning Cell-Tak 5 mg N/A - 354241 Corning Cell-Tak 10 mg N/A - Cat. No. Packaging € 003535 10 mg N/A - Used to adhere cells or tissue fractions to all types of substrates (e.g. plastic, glass, Teflon and biological materials). hEasy to use hFor plastic and glass surfaces hIrradiated hQuality tested for batch-to-batch consistency hStore at -20 °C Easy to use self-coating material to create a unique synthetic surface that mimics the natural cellular environment. This surface supports the adhesion and expansion of stem cells in their undifferentiated state and the directed differentiation into specialised cell types. PAN BIOTECH range - Attachment factors - Extra-cellular matrices Cat. No. Description Vol. € P06-20030 Collagen A 1 x (6 x 5 ml) N/A - P06-20166 Collagen R (type I) sterile 0.2% solution 20 ml N/A - P06-20410 Gelatin solution 0.1% PBS 500 ml N/A - P06-25200 Gelatin solution 2% PBS 100 ml N/A - 2705005 Fibronectin 5 mg N/A - 27050019 Fibronectin solution 1 mg/ml N/A -
Cell Culture 14 h The use of "Boyden chambers", which can be found under different names as culture inserts (different products in the following pages), a method which consists of placing cells in a support which is superimposed on a culture plate, and observing whether the cells manage to pass through a porous membrane which has been selected according to the cells or the analyses to be carried out h The use of "wound test", "wound healing test" , which can be found under the name "scratch assay" (different products for this application in our catalogue: GREINER M3D, SPL…). A method for creating a cell-free zone in the middle of a confluent culture and observing whether and how the cells come together. Culture inserts 1) Define pore size: Depending on your kind of Analysis, the choice of pore size is important hCoCulture: 0.4 et 1 µm hCellular Migration: 3, 5 or 8 µm hCell Invasion, 3, 5 or 8 µm hTransport and permeability Analysis: 0.4 or 1 µm hToxicity Analysis: 0.4, 1 or 3 µm hEpithelial cell polarity: 0.4 µm hAngiogenesis: 3 or 8 µm 2) Define the type of membranes Choose the best model according to optical properties, cell attachment or exchange maximisation.. hPolycarbonate (PC) membrane: very high pore density, which maximises exchange by optimal diffusion. Translucent membrane makes observation by light microscopy more difficult hPolyester (PE) or polyethylene terephthalate (PET) membrane: pore density allowing transparency (except in certain specific cases) and ideal visualisation under microscopy hPolytetrafluoroethylene (PTFE) membrane: membrane that can be coated with a cell matrix and retains optical properties for microscopic analysis 3) Define the format The choice is made according to the cell type and the quantity of samples available. hPlates 6, 12, 24, 96 or Petri dishes 100 mm 4) Choose your surface treatment hCoated plates are used either to promote the adhesion of certain cell types, or to highlight certain phenomena such as cell invasion, it is time to choose the most suitable coating On the following pages, you will find tables that summarise all these characteristics in order to make it easier for you to find your way around Our team of specialists is also at your disposal, if you wish to discuss technical issues and get advice before making your choice Many diseases, such as cancer, arthritis or atherosclerosis, are consequences of complex processes at the cellular level. Cell migration and invasion are part of these processes. Cell migration refers to the movement of cells, or tissues, in physiological or pathological contexts. Cell invasion refers to a movement related to cell migration, where the cells have acquired an additional characteristic that allows them to cross the extracellular matrix. They have acquired the ability to degrade it. In order to avoid the progression of diseases by finding suitable drugs it is essential to study these two mechanisms with different tests : How to choose your culture inserts? How to tell the difference between Migration and Cell Invasion Migration Invasion Culture Insert Cells Matrix Insert membrane Receiving plate Culture medium Culture Insert Cells Matrix Insert membrane Culture Insert Cells Insert membrane Receiving plate Culture medium Receiving plate Culture medium
Cell Culture 15 All prices are indicative and exclude VAT. Visit www.dulis.nl Falcon® Inserts Falcon® Inserts: different formats h Falcon® individual inserts for 6, 12 or 24 well culture plates hPET membrane for easy cell adhesion and visualisation hUse with Companion plates designed for perfect centering of the insert in the well and optimal gas exchange hIndividually blister-packed, without plate hHigh density membrane (more pores) for optimum diffusion between the upper and lower well compartment h Falcon® integral inserts in 24 or 96 well plates h Test automation (robot compatible) hSupplied with a receiver plate for media renewal hFits Falcon® 24 and 96 plate sizes h Applications hPermeability of molecules analysis hCellular transport analysis hMeasuring of migration or invasion hChemotaxis Analysis Cat. No. Description Membrane type Membrane material Units/ box €/ box Porosity 0.4 µm 353090 Individual inserts for 6-well plates Transparent PET 48 N/A - 353493 Individual inserts for 6-well plates High density translucent PET 48 N/A - 353180 Individual inserts for 12-well plates Transparent PET 48 N/A - 353494 Individual inserts for 12-well plates High density translucent PET 48 N/A - 353095 Individual inserts for 24-well plates Transparent PET 48 N/A - 353495 Individual inserts for 24-well plates High density translucent PET 48 N/A - Porosity 1 µm 353102 Individual inserts for 6-well plates Transparent PET 48 N/A - 353103 Individual inserts for 12-well plates Transparent PET 48 N/A - 353104 Individual inserts for 24-well plates Transparent PET 48 N/A - 351181 24-well Insert System Transparent PET 5 N/A - 351131 96-well Insert System Transparent PET 5 N/A - Porosity 3 µm 353091 Individual inserts for 6-well plates Transparent PET 48 N/A - 353092 Individual inserts for 6-well plates High density translucent PET 48 N/A - 353181 Individual inserts for 12-well plates Transparent PET 48 N/A - 353292 Individual inserts for 12-well plates High density translucent PET 48 N/A - 353096 Individual inserts for 24-well plates Transparent PET 48 N/A - 353492 Individual inserts for 24-well plates High density translucent PET 48 N/A - 351183 24-well Insert System Transparent PET 5 N/A - Porosity 8 µm 353091 Individual inserts for 6-well plates Transparent PET 48 N/A - 353092 Individual inserts for 6-well plates High density translucent PET 48 N/A - 353181 Individual inserts for 12-well plates Transparent PET 48 N/A - 353292 Individual inserts for 12-well plate High density translucent PET 48 N/A - Accessories Cat. No. Description Units/ bag Units/ box €/ box 353502 Companion cell culture plate for individual 6-well inserts 1 50 N/A - 355467 Deep Well cell culture plate to accommodate individual 6-well inserts 1 4 N/A - 353503 Companion cell culture plate for individual 12-well inserts 1 50 N/A - 353504 Companion cell culture plate for individual 24-well inserts 1 50 N/A - 353925 Plate with square wells to accommodate one 96-well insert plate with lid 5 5 N/A - 351186 Reservoir plate to accommodate the 24-well insert plate with lid 5 5 N/A - 353924 Reservoir plate to accommodate the 96-well insert plate with lid 5 5 N/A - 353938 Reservoir plate for 96-well insert plate 5 5 N/A - 355467
Cell Culture 16 Corning® -Transwell® Inserts Corning® Inserts: different formats h Corning® individual inserts with 6, 12 or 24 well culture plates hDifferent types of membranes, facilitating the adhesion of all cell types hInserts delivered positioned in the plates (attention: 12 inserts in a 24-well plate) hSome inserts are coated to improve adhesion hPossibility to have a Fluoroblok membrane on some items hTranswell® technology has been widely published hPolycarbonate: maximum diffusion for drug transport, secretion or absorption studies h Corning® insert system in 24 or 96 well plates hTest automation (robot compatible) hPossibility of having a Fluoroblok™ membrane for certain items hSome inserts are coated to improve adhesion hInvasion, migration and angiogenesis chambers already prepared with suitable coatings h Fluoroblok™ technology hInserts with an opaque membrane that blocks the transmission of visible light between 400 and 700 nm hThe fluorescent cells in the upper part of the insert are thus no longer visible hThe labelled cells that have crossed the membrane will be the only ones detected by fluorometry: by reader or microscope hAvoids wasting time and the use of cotton swabs hEnables migration or invasion kinetics to be established without destroying the insert Cat. No. Description Membrane type Membrane material Units/ box €/ box Porosity 0.4 µm 003412 Individual inserts with 6-well plate Translucent PC 24 N/A - 003401 Individual inserts with 12-well plate Translucent PC 48 N/A - 003413 Individual inserts with 24-well plate Translucent PC 48 N/A - 003450 Individual inserts with 6-well plate Transparent PET 24 N/A - 003460 Individual inserts with 12-well plate Transparent PET 48 N/A - 003470 Individual inserts with 24-well plate Transparent PET 48 N/A - 003491 Individual inserts with 6-well plates with collagen coating Translucent PTFE 24 N/A - 003493 Individual inserts with 12-well plate with collagen coating Translucent PTFE 24 N/A - 003495 Individual inserts with 24-well plate with collagen coating Translucent PTFE 24 N/A - 003396 24-well Insert System Translucent PC 2 N/A - 003397 24-well Insert System Translucent PC 12 N/A - 003379 24-well Insert System Transparent PET 2 N/A - 003378 24-well Insert System Transparent PET 12 N/A - 003381 96-well Insert System Translucent PC 1 N/A - 003391 96-well Insert System Translucent PC 5 N/A - 007369 96-well Insert System Transparent PET 5 N/A - 354444 Biocoat™ Individual inserts for 24-well plates with collagen I coating Transparent PET 24 N/A - Porosity 1 µm 003380 96-well Insert System Transparent PET 1 N/A - 003392 96-well Insert System Transparent PET 5 N/A - 354474 Individual inserts for Biocoat™ 24-well plates with fibrillar collagen coating Transparent PET 24 N/A - Porosity 3 µm 003414 Individual inserts with 6-well plate Translucent PC 24 N/A - 003402 Individual inserts with 12-well plate Translucent PC 48 N/A - 003415 Individual inserts with 24-well plate Translucent PC 48 N/A - 003420 Petri dish inserts 100 mm Translucent PC 12 N/A - 003452 Individual inserts with 6-well plate Transparent PET 24 N/A - 003462 Individual inserts with 12-well plate Transparent PET 48 N/A - 003472 Individual inserts with 24-well plate Transparent PET 48 N/A - 003492 Individual inserts with 6-well plate with collagen coating Translucent PTFE 24 N/A - 003494 Individual inserts with 12-well plate with collagen coating Translucent PTFE 24 N/A - 003496 Individual inserts with 24-well plate with collagen coating Translucent PTFE 24 N/A - 003398 24-well Insert System Translucent PC 2 N/A - 003399 24-well Insert System Translucent PC 12 N/A - 003385 96-well Insert System Translucent PC 2 N/A - 003386 96-well Insert System Translucent PC 8 N/A - 351151 Individual inserts for 24-well plate Fluoroblok™ PET 48 N/A - 351156 24-well Insert System Fluoroblok™ PET 5 N/A - 351161 96-well Insert System Fluoroblok™ PET 1 N/A - 351162 96-well Insert System Fluoroblok™ PET 5 N/A - 354597 Individual inserts for Biocoat™ 24-well plates with fibronectin coating Fluoroblok™ PET 24 N/A -
Cell Culture 17 All prices are indicative and exclude VAT. Visit www.dulis.nl Corning® - Transwell® Inserts Corning® inserts: different formats (continued) Cat. No. Description Membrane type Membrane material Units/ box €/ box Porosity 3 µm 354142 Biocoat™ 24-well insert plate with matrigel coating angiogenesis endothelial cell invasion Fluoroblok™ PET 5 N/A - 354144 Biocoat™ 24-well insert plate with fibronectin coating endothelial cell migration Fluoroblok™ PET 5 N/A - 354144 Biocoat™ 24-well insert plate with fibronectin coating endothelial cell migration Fluoroblok™ PET 5 N/A - 354148 Biocoat™ 24-well insert plate with fibronectin coating endothelial cell migration Fluoroblok™ PET 5 N/A - Porosity 5 µm 003421 Individual inserts with 24-well plate Translucent PC 48 N/A - 003387 96-well Insert System Translucent PC 8 N/A - 003388 96-well Insert System Translucent PC 2 N/A - Porosity 8 µm 003428 Individual inserts with 6-well plate Translucent PC 48 N/A - 003422 Individual inserts with 24-well plate Translucent PC 48 N/A - 003464 Individual inserts with 24-well plate Transparent PET 48 N/A - 003374 96-well Insert System Transparent PET 2 N/A - 003384 96-well Insert System Transparent PET 8 N/A - 351152 Individual inserts for 24-well plate Fluoroblok™ PET 48 N/A - 351157 24-well Insert System Fluoroblok™ PET 1 N/A - 351158 24-well Insert System Fluoroblok™ PET 5 N/A - 351163 96-well Insert System Fluoroblok™ PET 1 N/A - 351164 96-well Insert System Fluoroblok™ PET 5 N/A - 354481 Biocoat™ Individual insert for 6-well plate with matrigel coating invasion chamber Transparent PET 24 N/A - 354480 Biocoat™ Individual insert for 24-well plate with matrigel coating invasion chamber Transparent PET 24 N/A - 354483 Biocoat™ Individual insert for 24-well plate with matrigel coating. Reduces growth factor invasion chamber Transparent PET 24 N/A - 354165 Biocoat™ 24-well insert plate with matrigel coating invasion chamber Fluoroblok™ PET 1 N/A - 354166 Biocoat™ 24-well insert plate with matrigel coating invasion chamber Fluoroblok™ PET 5 N/A - 354167 Biocoat™ 96-well insert plate with matrigel coating invasion chamber Fluoroblok™ PET 1 N/A - 354168 Biocoat™ 96-well insert plate with matrigel coating invasion chamber Fluoroblok™ PET 5 N/A - Accessories Accessories Snapwell™ inserts Netwell™ PET inserts Cat. No. Description Units/ bag Units/ box €/ box 003395 Reservoir for 24-well insert plate, non treated 12 48 N/A - 003382 Reservoir plate to hold the 96-well insert plate with culture treatment 10 10 N/A - 003383 Reservoir plate to hold the 96-well insert plate with media stabiliser 10 10 N/A - 003583 Black reservoir plate to hold the 96-well insert plate with culture treatment 10 10 N/A - 003783 White reservoir plate to hold the 96-well insert plate with culture treatment 10 10 N/A - 353928 Reservoir plate with square wells to hold the fluoroblok 96-well insert plate 5 5 N/A - 354216 Calcein AM Fluorescent Fluorophores Marker - 10 x 50 µg N/A - 354217 Fluorescent Calcein AM Fluorophores Marker - 1 mg N/A - 354218 Fluorophores Fluorescent marker DilC12(3) - 100 mg N/A - Cat.No. Description Units/ box €/ box 003517 Netwell™ black tray 200 N/A - 003519 Netwell™ white tray 200 N/A - 003520 12-well transport system for 15 mm inserts 8 N/A - 003521 6-well transport system for 24 mm inserts 8 N/A - Cat.No. Description Membrane material Units/ box €/ box 003407 Snapwell™ insert for 6-well plate PC 24 N/A - 003801 Snapwell™ insert for 6-well plate PET 24 N/A - Cat.No. Description Porosity (µm) Units/ box €/ box 003477 Individual inserts for 12-well plate 74 48 N/A - 003478 Individual inserts for 12-well plate 440 48 N/A - 003479 Individual inserts for 6-well plate 74 48 N/A - 003480 Individual inserts for 6-well plate 440 48 N/A - hThe Snapwell™ insert is a Transwell™ culture insert consisting of a 12mm membrane supported by a removable ring h2 types of membranes, polycarbonate or PET hOnce the cells have reached confluence, the ring can be placed in a vertical or horizontal diffusion chamber hFor electrophysiological or transport studies hSterile hPorosity: 0.4 µm hPolystyrene inserts with a polyester mesh bottom hUse as plates or sieves for the culture of small organs, tissue sections or explants at the airliquid interface hThey allow coarse filtration of tissue homogenates or cell suspensions hVery useful for immunohistochemical staining of tissue sections hSterile
RkJQdWJsaXNoZXIy MjM2OTUxOA==