125 sera media All prices are indicative and exclude VAT. Visit www.dulis.nl hSimple and efficient separation of PBMCs (lymphocytes and mononuclear cells) from blood or bone marrow hConcentration directly from whole blood hRapid separation in 15 minutes at room temperature hDuring centrifugation, lymphocytes and mononuclear cells are separated from unwanted elements by density gradient and concentrate in a phase above the separating medium hWhen separation is complete, at the time of harvest, the porous barrier minimizes contamination of the enriched fraction with red blood cells hPre-filled with LeucosepTM separation medium (density 1.077 g/ml) hAvailable unfilled for use with all other separation media on the market • A simple, fast and efficient system for the enrichment of circulating tumour cells released from solid tumours or malignant melanomas. • Consists of a polypropylene tube with a porous barrier inserted on top of a specially developed separation solution. • Up to 30 mI of the whole anticoagulated blood is deposited directly into the OncoQuickTM tube, and centrifuged. • Apart from erythrocytes and granulocytes, the separation medium allows the virtual elimination of lymphocytes and mononuclear cells. • Circulating tumour cells are concentrated at the interface. hResult in 45 minutes hFor research use only hReproducible recovery hBlood cell removal up to 6 log units hNo additional laboratory equipment hNo magnetic balls hNo blockage of marker molecules hEnrichment directly from whole blood Enrichment in circulating tumour cells disseminated from whole blood. Cat. No. Quantity € 490002 10 g N/A - 490003 50 g N/A - 490004 100 g N/A - hFor the separation of T leukocytes from B leukocytes hInstructions for use on request Filter held by two rings moulded into the tube. These ensure that the filter remains in place during centrifugation. Blood Sample preparation LeucosepTM 12 ml and 50 ml Tubes OncoQuickTM Greiner Bio-One System Blood Sep Filter Nylon wool Cat.No. Vol. (ml) LeucosepTM separation medium Sterile Units/ box Units/ bag €/ bag 163288 12 Yes Aseptic 50 500 N/A - 163289 12 No No 50 500 N/A - 016765 12 No Yes 50 500 N/A - 227288 50 Yes Aseptic 25 250 N/A - 016761 50 No No 25 300 N/A - 016760 50 No Yes 25 300 N/A - Cat. No. Total tube volume (ml) Blood volume (ml) Units/ box €/ box 227255 50 15 - 30 4 N/A - 227250 50 15 - 30 10 N/A - Cat. No. Total volume of Blood Sep Filter tube Volume of blood Units/ bag €/ bag 016780 50 ml without MSL 15 - 30 ml 25 N/A -
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