124 sera media Blood Sample preparation Ready-to-use and sterile solution based on FICOLL, a solution consisting of a polysaccharide with a molecular weight of 400 000 daltons. hFor the preparation of lymphocyte solutions from whole blood hCan be stored between 2 °C and room temperature hQuality certificate available hpH 6.5 - 7.5 for all types hPANCOLL Human density: 1.077 g/ml hOsmolarity: 280 - 300 mOSM/kg hPANCOLL Animal density: 1.077 g/ml hOsmolarity: 255 - 275 mOSM/kg hPANCOLL mouse, density 1.086 g/ml hOsmolarity: 270-290 mOSM/kg hPANCOLL mouse, density 1.068 g/ml hOsmolarity: 310-330 mOSM/kg hStimulates proliferation of lymphocytes in culture hAlso used for the separation of leukocytes from whole blood PANCOLL Human PANCOLL Animal PANCOLL Monocytes* Cat. No. Désignation Volume € L3010-005 Phytohaemagglutinin liquid 5 ml N/A - hReduces the risk of contamination hTime saving hStable 36 months at 2-20 °C hStore in a dark place Membrane Separation Medium PANCOLL Plasma Fraction lymphocytes PBMCs Erythrocytes Granulocytes Blood Before centrifugation tubes filled with PANCOLL (density 1.077 g/ml) After centrifugation tubes Pre-filled PANCOLL human MSL Pre-filled PANCOLL animal Cat. No. Désignation Tubes MSL volume € 016760F Rack of 25 tubes 50 ml 15 ml N/A - 016765F Rack of 50 tubes 10 ml 3 ml N/A - Cat. No. Désignation Tubes MSL volume € 016775F Rack of 50 tubes 10 ml 3 ml N/A - Cat. No. Désignation Density (g/ml) Volume (ml) Units/ box €/ box 17-5446-02 Ficoll-Paque Premium 1.084 1.084 100 6 N/A - 17-5446-52 Ficoll-Paque Premium 1.073 1.073 100 6 N/A - Cat. No. Désignation Volume (ml) Units/ box €/ box 17-0891-09 Percoll 1000 6 N/A - 17-5445-01 Percoll Plus 1000 1 N/A - 17-5445-02 Percoll Plus 250 1 N/A - Cat. No. Désignation Weight € 17-0300-05 Ficoll PM 400 5 kg N/A - 17-0300-10 Ficoll PM 400 100 g N/A - 17-0300-50 Ficoll PM 400 500 g N/A - 17-0310-10 Ficoll PM 70 100 g N/A - 17-0310-50 Ficoll PM 70 500 g N/A - Ficoll-Paque Premium hReady-to-use solution for the separation of mononuclear cells from blood hConsists of a sucrose polymer hSterile Ficoll powder Percoll hConsists of colloidal sillice particles surrounded by PVP (Percoll) or silane (Percoll Plus) hDensity: 1.130 g/ml hSuitable for separation of polynuclei from neutrophils Cat. No. Corning Cat. No. Désignation Vol. (ml) € 702627 25-072-CI LSM medium - Density 1077-1080 g/ml 100 N/A - 702628 25-072-CV LSM medium - Density 1077-1080 g/ml 500 N/A - Cat. No. Désignation Vol. (ml) € L0560-100 Lymphocyte separation medium 100 N/A - L0560-500 Lymphocyte separation medium 500 N/A - hOsmolarity 290 + 20 mOsm hLow viscosity: in vitro isolation of lymphocytes from whole blood Lymphocyte separation media PANCOLL PANCOLL Mouse Lymphocyte separation medium (LSM) Lymphocyte separation media Leucosep "ready to use" tubes Ficoll and Percoll density gradient PHA-M (Phytohaemagglutin) Cat. No. Density Osmolarity pH Volume (ml) € 500201 1.077 g/ml 280-300 6.5 - 7.5 100 N/A - 500205 1.077 g/ml 280-300 6.5 - 7.5 500 N/A - Cat. No. Density Osmolarity pH Volume (ml) € P04-63100 1.077 g/ml 255-275 6.5 - 7.5 100 On request P04-63500 1.077 g/ml 255-275 6.5 - 7.5 500 On request Cat. No. Density Osmolarity pH Volume (ml) € P04-64100 1.086 g/ml 270-290 6.5 - 7.5 100 On request P04-64500 1.086 g/ml 270-290 6.5 - 7.5 500 On request Cat. No. Density Osmolarity pH Volume (ml) € P04-68100* 1.068 g/ml 325-355 6.5 - 7.5 100 On request P04-68500* 1.068 g/ml 325-355 6.5 - 7.5 500 On request *Cat. No. on request
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